Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTLIILTVLLLSVLTAACSSSSGNQNSKEHKVAVTHDLGKTNVPEHPKRVVVLELGFIDTLLDLGITPVGVADD--NKAKQLINKDVLK--KIDGYTSVGTRSQPSMEKIASLKPDLIIADTTRHKKVYDQLKKIAPTIALNNLNADYQDTIDASLTIAKAVGKEKEMEKKLTAHEEKLSETKQKISANSQ-------SVLLIGNTNDTIMARDENFFTSRLLTQVGYRYAISTSGNSDSSNGGDSVNMKMTLEQLLKTDPDVIILMTGKTDDLDADGKRPIEKNVL----WKKLKAVKNGHVY-HVDRAVWSLRRSVDGANAILDELQKEMPAAKK 3MWF Chain:A ((7-291)) ----------------------------------SIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKI-----VGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTV-FKFKDT---TKLMGKALGKEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYA---GGYAGEILNDLGFKRNKDLQKQVD--NGKDIIQL-TSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKL-----

General information: TITO was launched using: RESULT: Template: 3MWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1443 -94855 -65.73 -352.62 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -65.73 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3MWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MWF-query.scw PDB file : Tito_Scwrl_3MWF.pdb: