Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSKLIFFDIDGTIYDHDKN-IPESTRKTVAELQRQGHHVFIASGRSPFLVKPILEELG----IHSFISYNGQFVVFENQVIYKNPLPENAIRRLLKQADEGKHPVVFMAEDTMKATVADHPHVLEGIGSLKTDYPETDDL----FYEG--KEIFQLLLFCQDEEEK-AYAAFPEFDLVRWHELSTDVLPHGGSKAEGIKKVIERLPFDIGDTYAFGDGLNDLQMIEYVGTGVAMGNAVPELKEIADFVTKPVDEDGIAYAVKELGLLK 2RAV Chain:A ((2-261)) --TKALFFDIAGTLVSFETHRIPSSTIEALEAAHAKGLKIFIATGRPKAIINN-LSELQDRNLIDGYITMNGAYCFVGEEVIYKSAIPQEEVKAMAAFCEKKGVPCIFVEEHNISVC---QPN--EMVKKIFYDFLHVNVIPTVSFEEASNKEVIQMTPFITEEEEKEVLPSIPTCEIGRWYPAFADVTAKGDTKQKGIDEIIRHFGIKLEETMSFGDGGNDISMLRHAAIGVAMGQAKEDVKAAADYVTAPIDEDGISKAMKHFGII-

General information: TITO was launched using: RESULT: Template: 2RAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1535 -181542 -118.27 -732.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -118.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_2RAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RAV-query.scw PDB file : Tito_Scwrl_2RAV.pdb: