Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVIQVKGNVTYPITIDPSVWIFDDRKFSFDRKGDSQDTYLQSAGDESDLDPERVIREGRIAPPTLKTEKQYEKQKLMNGSFAMRLGTVLKNAEPNSSATQCVFVTSS-----G-KAAVSLETAL----NSIVHFSEAGKPIQ--EGGPVHIYFEDPVHHKQPITDVKEIEII 2BIH Chain:A ((159-249)) ---------------------------------------------------------------------------------TGCMLGDVIGKARPSKRARFVWMEGADNPANGAYGTCIRLSWCMDPERCIMIAYQQNGEWLHPDHGKPLRVVIPGVIGGRS-VKWLKKLVVS

General information: TITO was launched using: RESULT: Template: 2BIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 345 -65206 -189.00 -825.39 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -189.00 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.700

(partial model without unconserved sides chains): PDB file : Tito_2BIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BIH-query.scw PDB file : Tito_Scwrl_2BIH.pdb: