Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEKKEIATFAGGCFWCMVKPFDEQPGIEKVVSGYTGGHTENPTYEEVCSETTGHREAVQITFHPDVFPYEKLLELFWQQIDPTDAGGQFADRGSSYRAAIFYHNDKQKELAEASKQRLAESGIFKDPIVTDILKAEPFYEAEGYHQHFYKKNPAHYQRYRTGSGRAGFISEHWGAK 2J89 Chain:A ((94-246)) ----QQFAQFGAGCFWGVELAFQRVPGVTKTEVGYTQGLLHNPTYEDVCTGTTNHNEVVRVQYDPKECSFDTLIDVLWARHDPTTLNRQGNDVGTQYRSGIYYYTPEQEKAAKESLER--QQKLLNRKIVTEILPAKKFYRAEEYHQQYLAK-----------GGRFGFM-------

General information: TITO was launched using: RESULT: Template: 2J89.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 750 -49631 -66.17 -324.39 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -66.17 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2J89.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J89-query.scw PDB file : Tito_Scwrl_2J89.pdb: