Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQEKAIIIGGGPCGLSAAIHLKQIGIDALVI---EKGNVVNS---IYNYPTHQTFFSSSEKLEIGDVAFITENRKPVRIQALSYYREVVKRKNIRVNAFEMVRKVTKTQNNTFVIETSKETYTTPYCIIATGYYDHPNYMGVPGEDLPK----VFHYFKEGHPYFDKDVVVIGGKNSSVDAALELVKSGARVTVLYRGNEYSPSIKPWILPEFEALVRNGTIRMEFGACVEKITENE-----VVFRSGEKE--LITIKNDFVFAMTGYHPDHQFLEKIGVEIDKETG----RPFFNEETMETNVEGVFIAGVIAAGNNANEIFIENGRFHGGHIAAEIAKRENH 1TDF Chain:A ((5-285)) --HSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQLTTTTEVENWPGDPNDLTGPLLME------------RMHEHATKFETEII---------FDHINKV-DLQNRPFRLNGDNGEYTCDALIIATGA--SARYLGLPSEEAFKGRGVSACATSDGFFYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGFRA--EKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGVTGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEG---QLELENGYIKVQSGIHGNATQTSIPGVFAAG---------------------------------

General information: TITO was launched using: RESULT: Template: 1TDF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1332 32102 24.10 123.47 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 24.10 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1TDF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TDF-query.scw PDB file : Tito_Scwrl_1TDF.pdb: