Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIIAGTVVKGK-QLGRKLGFPTAN------VDAKIHGLRNGVYGVLATV--------NHQFHLGVMNIGVKPTVGSNLEKTLEIFLFDFHRDIYGEKIECSILFKIREERRFDSLESLTKQIKKDISCVAKRFELIGIMAPNKKESLLSHQELNLPDLCFYKKCNNLYGVNRGVYNVIDNWFFEYGITQVAYRRIYILSFLSFLKEDNPKVSSKYIRFGAGGLADKLNRFISSYVEESEENILG 5A8A Chain:A ((7-129)) ---VTGPVVRGAGRGGKELGFPTANQYFHDTVALPADGVYAGWLTILPTEAPVSGNMEPEVAYAAAISVGTNPTFGDE-QRSVESFVLDRDADLYGHDVKVEFVDHVRAMEKFDSVEQLLEVMAKDV----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5A8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 492 26786 54.44 248.01 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 54.44 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_5A8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A8A-query.scw PDB file : Tito_Scwrl_5A8A.pdb: