Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYNIRGENIEVTPALKDHVERKIGKLERYFDHSVDADVNVNLKFYNDKESK---VEVTIPMTDLALRSEVHNEDMYNAIDLATNKLERQIRK--HKTKVNRKFREQGSPKYLLANGLGSDTDIAVQDDIEEEESLDIVRQKRFNLKPMDSEEAILQMNMLGHNFFVFTNAETNLTNVVYRRNDGKYGLIEPTE 1N3G Chain:A ((1-96)) MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILS------KEPQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARR--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1N3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 402 7072 17.59 77.71 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 17.59 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_1N3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N3G-query.scw PDB file : Tito_Scwrl_1N3G.pdb: