Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSHIYKKEMIDALRDRKTILLTILVPMIMMLGLVFFYESMLSDKGEQYTLAVGHSLPPALESKLNEI--DEISV--KTFAKPEEAVDE-GKADAYLNVPKEFDSYVNSMTP-FKVDVYGNSIDQGSSNAMQLVQSALDQYKNEIVQQRLTNKHIDQSVIQPFTIQQKEADEEKGTSAIMLSAILPMLILTSIVSGAMPIALDIMAGEKDRKSIEALLLTPVSRNKVLVGKWLAVSTFGVASGVFALVFLILSTVLFTENLKTAFQLGDHMWSVIGASALIIVLSALLISAMELFISIMSSSVKEAQSYMSLVVFLPVFPMFFIFSKAPNQFDLSYFLIPFLNLHALFKQLLFGMVDPATILSTSGTIAVLIAIFFLLARACFLKDKWVLPK 2P0S Chain:A ((8-100)) ----------------------------------------------DMKTIAIADRTGE-YEQLFKENDEFRFVHAEKTAEEYRKMGADKSGIDAVLEIRQDLL------EDPNAVAIYGYKQLPA--SVSNHISRILSDYL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 313 -32749 -104.63 -404.31 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -104.63 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_2P0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P0S-query.scw PDB file : Tito_Scwrl_2P0S.pdb: