Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAISLEKGQRIDLTKGKAGLSKLMVGLGWDPVSSGGGFFSKLLGGGGPNIDCDASVLMLE-NGKFTDKKNLIYFGNLKSRCGGVQHTGDNLTGDGAGDDEQIMIDLDKVPGNIDKLVFVVNIYDCVRRKQDFGMIQNAFIRVVDQSNHEEMLKYNLRDNYAGRTSLITAEIYRS-GSEWKFAAVGEGTNDTRLEDIISRYV 2QZ7 Chain:A ((22-186)) ------------------GLKRVDVRLKWDPSPWD-----------RPPHHLDIIATTYAADAPHGRPVYVVQFD-KRSPDGTINMSRHSRTGQGFGFVEEMTFELDRLSPSIARVIVGVAIHQDN-GHKTFDDVSNTGVVVAEG--YRELLTDGFE-RVAGATAATVAEFTRNASGAWEFREAVRGFDS-DPVLFATEM-

General information: TITO was launched using: RESULT: Template: 2QZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 891 -97535 -109.47 -598.37 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -109.47 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_2QZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QZ7-query.scw PDB file : Tito_Scwrl_2QZ7.pdb: