Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKIIGIGVSAMLALSLAACGSENDENASAAEQVNKTIIGIDPGSG----IMSLTDKAMKDYDLNDWTLISASSAAMTATLKKSYDRKKPIIITGWTPHWMFSRYKLKYLDDPKQSYG---SAEEIHTITRKGFSKEQPNAAKLLSQFKWTQDEMGEIMIKV-EEGEKPAKVAAEYVNKHKDQIAEWTKGVQKVKGDKINLAYVAWDSEIASTNVIGKVLEDLGYEVTLTQVEAGPMWTAIATGSADASLSAWLPNTHKAYAAKYKGKYDDIGTSMTGVKMGLVVPQYMKNVNSIEDLKK 2REG Chain:A ((112-278)) ----------------------------AAHKDELDGKIYGIEPGNDGNRLIIDMVEKGT--FDLKGFEVVESSEQGMLAQVARAEKSGDPIVFLGWEPHPMNANFKLTYLSGGDDVFGPNYGGATVHTNVRAGYTTECPNVDKLLQNLSFSLQMENEIMGKILNDGEDPEKAAAAWLKDNPQSIEPWLSGVATKDG--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2REG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 -43380 -93.29 -272.83 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -93.29 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_2REG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2REG-query.scw PDB file : Tito_Scwrl_2REG.pdb: