Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTEN-GIHIESI-----MSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV 2AEU Chain:A ((275-364)) ---------------------------------EKLNKELKAIDDNINIVYERTPTGFVIKRVYKDDTIN-IKKLIEIGFNLLKNYGIITITVAGMPGASKSLRIDLTSRD---------AERID---DNYIIKAI--

General information: TITO was launched using: RESULT: Template: 2AEU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 284 -28745 -101.21 -342.20 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -101.21 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_2AEU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AEU-query.scw PDB file : Tito_Scwrl_2AEU.pdb: