Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLFKSFDASEKTQLICFPFAGGYSASFRPLHAFLQGECEMLAAEPPGHGTNQTSAIEDLEELTDLYKQELNLRP---DRPFVLFGHSMGGMITFRLAQKL-EREGIFPQAVIISAIQPPHIQR----KKVSHLPDDQFLDHIIQLGGMPAELVENKEVMSFFLPSFRSDYRALEQF--ELYDLAQIQSPVHVFNGLDDKKCIRDAEGWKK-WAKDITFHQFDGGHMFLLSQTEEVAERIFAILNQHPIIQP 4XJV Chain:A ((31-252)) ---------ATFK--LICFPWMGGGSTHFAKWGQDTHDLLEVHSL-----------------QLVD--EVVCALQPVIQDKPFAFFGHSMGSYIAFRTALGLKENNQPEPLHLFLSSATPVHSKAWHRIPKDDELSEEQISHYLMEF-------AEAKEFVKQCSPIIRADLNIVRSCTSNVPSKAVLSCDLTCFVGSED--IAKDMEAWKDVTSGNAKIYQLPGGHFYLLDPANE-----------------

General information: TITO was launched using: RESULT: Template: 4XJV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 878 -123036 -140.13 -661.48 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -140.13 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_4XJV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XJV-query.scw PDB file : Tito_Scwrl_4XJV.pdb: