Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKAAVRNMRLEDIDHVYEIEASSFTSPWTKDSFYHELLENPYAHYLVIEKDGHLAGYCGIWI------------VMDDAQITNIAIKPEYRGQSLGETLFRSAVELCK-EKDARRLSLEVRVSNHPAQGLYKKFGMQPGGIRKNYYTDNGEDALIMWVTINE 5C88 Chain:A ((14-163)) ------LRNARMDDIDQIIKINRLTLPENYPYYFFVEHLKEYGLAFFVAI-VDNSVVGYIMPRIEWGFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNFKKVKVLKGYYAD-GEDAYLM------

General information: TITO was launched using: RESULT: Template: 5C88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 610 -92990 -152.44 -678.76 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -152.44 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_5C88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C88-query.scw PDB file : Tito_Scwrl_5C88.pdb: