Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIIGRFKDIMSANINALLDKAENPEKMVDQYLRNMNSDLAKVKAETAAVMAEEQRAKREYHECQADMEKMESYAMKALQAGNESDARKFLERKTSLESKLSELQAANQIAATNAAQMRKMHDKLVSDIGELEARKNMIKAKWAVAKTQERMNKLGASVSSTSQSMSAFGRMEDKVNKALDQANAMAELNSAPQDDMADLSAKYDTGGSSQVDDELAALKAKMMLDK 3HIZ Chain:B ((458-567)) -------------------EKSREYDRLYEEYTRTSQ----EIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRR-------RLEEDLKKQAAEYREIDKRMNSIK---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HIZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 161 -8719 -54.15 -79.26 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -54.15 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3HIZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HIZ-query.scw PDB file : Tito_Scwrl_3HIZ.pdb: