Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIK-RLLDYVEDSDLADVPDPYY--TGNFEEVCQLIKTGCEQLLASIQKEKQL 4Z9B Chain:A ((14-160)) --SVLFVCLGNICRSPIAEAVFRKLVTDQNISENWRVDSAATSGYEIGNPPDYRGQSCMKRHGIPM-SHVARQITKEDFATFDYILCMDESNLRDLNRKSNQVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFL---------

General information: TITO was launched using: RESULT: Template: 4Z9B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 761 -92559 -121.63 -642.77 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -121.63 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_4Z9B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z9B-query.scw PDB file : Tito_Scwrl_4Z9B.pdb: