Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNLIYNEWLKIFSRAGTWVMIGILGLTMVGFAFLANHFSAGESNSHWKQELQAQNAELKKEIKEDPSLKDGYKETITLNDYRIEHNIPSDTGYTVWSYVTDSANFTILTGLFTIIIAAGIVANEFNWGTIKLLMIRPLSRFQILMSKYITVLLFGLLLLLILFIGSTLLGLIFFGTGGETAANIHLIYKDGHVIEQNMMGHLATTYLSESVSALMVATMAFMLSAVFRNSSLAVGFSIFLLVAGTTATAFIAAKFDWAKYILFANVDLTQYVDGTPLIKGMTMTFSLVMLAIYFIIFLLLAFGIFMKRDIAN 1IFL Chain:A ((33-48)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTVVVAGLVIRLFKKF----

General information: TITO was launched using: RESULT: Template: 1IFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 30 30.00 1.88 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.40 3D Compatibility (PKB) : 30.00 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_1IFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IFL-query.scw PDB file : Tito_Scwrl_1IFL.pdb: