Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGTSSRSTKLVHGGLRYLKQFEVKMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFTTSIGLRVYDFLAGVKKSERRSMLSAKETLQ-KEPLVKKDGLKGGGYYVEYRTDDARLTIEVMKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLREKD-HSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGK-TYVGTTDTVYKEALEHPRMTTEDRDYVIKSINYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKDEIWTSD----SGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGEKDFGPCKTKNMPISGGHVGGSKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHILAEAE--YSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTENLNKLLHDAVVPLEQ 2R46 Chain:A ((27-471)) ---------------------------------------------LSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLP-HRPHLRPAWMIRIGLFMYDHLG------KRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWI-VEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPR-VHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKK-QLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYY-----QGIGPAWTKESVLPGGAIEGDRDDYA---ARLRRRYPF-LTESLARHYARTYGSNSELLLGNAGTVSDLGEDFG---HEFYEAELKYLVDHEWVRRADDALWRRT----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2R46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2283 -106319 -46.57 -243.85 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -46.57 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_2R46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R46-query.scw PDB file : Tito_Scwrl_2R46.pdb: