Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD 1FMA Chain:E ((24-137)) --------------------ERDEDGAVVTFTGKVR-------VNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWV-----------------------

General information: TITO was launched using: RESULT: Template: 1FMA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 449 -57020 -126.99 -532.90 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain E : 0.75 3D Compatibility (PKB) : -126.99 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_1FMA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FMA-query.scw PDB file : Tito_Scwrl_1FMA.pdb: