Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEIGDDRG-RIDLVNPEILEKSGEQTGI-EGCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG 2W3U Chain:A ((1-147)) -SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGE-TGIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLS-------------

General information: TITO was launched using: RESULT: Template: 2W3U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 736 -69749 -94.77 -481.03 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -94.77 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2W3U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W3U-query.scw PDB file : Tito_Scwrl_2W3U.pdb: