Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTIHITHPHHLIKEEQAKSVMALGYFDGVHLGHQKVIGTAKQIAEEKGLTLAVMTFHPHPSHVLGRDKEPKDLITPLEDKINQIEQLGTEVLYVVKFNEVFASLSPKQFIDQYIIGLNVQHAVAGFDFTYGKYGKGTMKTMPDDLDGKAGCTM--VEKLTEQDKKISSSYIRTALQNGDVELANVLLGQPYFIKGIVIHGDKRGRTIGFPTANVGLNNSYIVP-PTGVYAVKAEV-NGEVYNGVCNIGYKPTFYEKRPEQPSIEVNLFDFNQEVYGAAIKIEWYKRIRSERKFNGIKELTEQIEKDKQEAIRYFSNLRK 1T6X Chain:A ((2-277)) --------------------VVSIGVFDGVHIGHQKVLRTMKEIAFFRKDDSLIYTISYPPEYFL---PDFPGLLMTVESRVEMLSRYARTV--VLDFFRI-KDLTPEGFVERYLSG--VSAVVVGRDFRFGKNASGNASFLR-----KKGVEVYEIEDVVVQGKRVSSSLIRNLVQEGRVEEIPAYLGRYFEIEGIV-----------FPTANIDRGNEKLVDLKRGVYLVRVHLPDGKKKFGVMNVGFR------R--NVKYEVYILDFEGDLYGQRLKLEVLKFMRDEKK----EELKAAIDQDVKSA---------

General information: TITO was launched using: RESULT: Template: 1T6X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1253 -164099 -130.96 -653.78 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -130.96 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1T6X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T6X-query.scw PDB file : Tito_Scwrl_1T6X.pdb: