Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHITTKRLLIREFEFKDWQAVYEYTSDSNVMKY-IPEGVFTEEDAKAFVNKNKGDNAEKFPVILRDEDCLIGHIVFYKY-FGEHTYEIGWVFNPNYQNKGYASEAAQAILEYGFKEMNLHRIIATCQPENIPSYRVMKKIGMRREGFFKKCIPKGNEWWDEYYYAILEEEWN 4JLY Chain:A ((5-146)) ----NSQLTLRALERGDLRFIHNLNNNRNIMSYWFEEPYESFDELEELYNKHIHDNAERRFVVEDAQKNLIGLVELIEINYIHRSAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEG--------------------------

General information: TITO was launched using: RESULT: Template: 4JLY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 517 -80172 -155.07 -572.65 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -155.07 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.640

(partial model without unconserved sides chains): PDB file : Tito_4JLY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JLY-query.scw PDB file : Tito_Scwrl_4JLY.pdb: