Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMNEHRNRRLKLIMISATFGGLLFGYDTGVINGALPFMARPDQLDLT-----PVTEGLVTS-------ILLLGAAFGALLCGRLADRYGRRKMILNLSFLFFLASLGTAL---APNVFIMAVFRFLLGLAVGGASAMVPAFLAEMAPHEKRGRMVTQNELMIVGGQFLAYVFNA--ILGVTMANTGHVWRYMLVICAVPAIMLFASMLKVPESPRWL-ISKGKNSEALRVLKQIREDKRAEAECREIQEAVEKDTALEKAS-LKDFSTPWLRRLLWIGIGVAIVNQITGVNSIMYYGTQILKESGFGTKAALIANIGNGLISVIAVIFGIWLVGKVRRRPILLIGLAGTTTALLLIAIFSIVLDGSMALPYVVLSLTVLFLAFMQGCVGPVTWLVIAEIFPQRLRGLGSGISVFFLWILNFVIGFAFPILLSSVGLSFTFFIFVALGVLAIGFVYKFMPETKGRTLEELEEHFRSR-HDHNTPEQSVIEV 5C65 Chain:A ((17-469)) -----------------ATIGSFQFGYNTGVINAPEKIIKEFIQKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILG-----SEELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDA------PIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKD----MSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLG-AYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQA-------------

General information: TITO was launched using: RESULT: Template: 5C65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2222 -229408 -103.24 -539.78 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -103.24 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_5C65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C65-query.scw PDB file : Tito_Scwrl_5C65.pdb: