Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVIHNGKAGLLGLSVQDVPSTKPGYGEVKVKLKSAGLNHRDLFLMKNKSEQDP----HMILGSDGAGIIEEIGEGVKNVTVQTEVVIFPTLNWDLTENVPPVPEILGGPSDGTLAEYVIIPSQNAIKKPAYLSWEEAGVLPLSALTAYRALFTKGQLKKGEHLLIPGIGSGVATYALFMAKAIGATVSVTSRSEEKRKKALKLGADYAFDSYSNWD------EQLQGKKIDVVLDSIGPALFSEYFRHVKPNGRIVSFGASSGDNLSFPVRS-LFFPQVNVLGTSMGSGE-EFQAML--AFIDK----------HKLRPVIDRIYPLEKACEAYKRMQEGRQFGNIGIVME 2OBY Chain:A ((7-332)) MLAVHFDKPGGPENLYVKEVAKPSPGEGEVLLKVAASALNRADL--MQRQGQYDPPPGASNILGLEASGHVAELGPGCQG-------------HWKIGDTAM---ALLPG---GGQAQYVTVPEGLLMPIPEGLTLTQAAAIPEAWLTAFQLLHLVGNVQAGDYVLIHAGLSGVGTAAIQLTRMAGAIPLVTAGSQKKLQMAEKLGAAAGFN-YKKEDFSEATLKFTKGAGVNLILDCIGGSYWEKNVNCLALDGRWVLYGLMGGGDINGPLFSKLLFKRGSLITSLLRSRDNKYKQMLVNAFTEQILPHFSTEGPQRLLPVLDRIYPVTEIQEAHKYMEANKNIGKI-----

General information: TITO was launched using: RESULT: Template: 2OBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1711 25680 15.01 85.03 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 15.01 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2OBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OBY-query.scw PDB file : Tito_Scwrl_2OBY.pdb: