Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIMSAFVGMVLLTIFCFSPQASAAYDDLHEGYATYTGSGYSGGAFLLDPIPSDMEITAINPADLNYGGVKAALAGSYLEVEGPKGKTTVYVTDLYPEGARGALDLSPNAFRKIGNMKDGKINIKWRVVKAPITGNFTYRIKEGSSRWWAAIQVRNHKYPVMKMEYEKDGKWINMEKMDYNHFVSTN-LGTGSLKVRMTDIRGKVVKDTIPKLPESGTSKAYTVPGHVQFPE 4JCW Chain:A ((10-179)) ---------------------------------YSLGQGNTIANGNCSMPAVPADRMYVAVSSPE--YSG--AAACGTFLDVTGPKGTVRVQVADQCHGCEVGHLDLSEEAFRALGDFNAGIIPISYVTVRDPAGPTVAIRVKEGSSRWWAGLQVLNAGNRIDRVEIQAGRQWLPLTRTDYGYWVTPSPIQDGPLTVKVTDQYGRAV--------------------------

General information: TITO was launched using: RESULT: Template: 4JCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 829 -59302 -71.53 -350.90 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -71.53 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_4JCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JCW-query.scw PDB file : Tito_Scwrl_4JCW.pdb: