Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKDKNKIIKSVEKINKLEEGLSLFEEGDEEYLSV-LVKIQGLYDEIS-----DTALECFKEMTTKIRKTGQKRIIKGIDQLPHTIKENIADQVNDFKGGAI 4OZS Chain:A ((6-66)) -----NTLIDGLAKAGRLEEALQLFQEMKEKGVKPDVVTYNTLIDGLAKAGRLEEALQLFQEMKEK------------------------------------

General information: TITO was launched using: RESULT: Template: 4OZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 168 -3767 -22.42 -68.49 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -22.42 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_4OZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OZS-query.scw PDB file : Tito_Scwrl_4OZS.pdb: