Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYESKTGNVKRFVK--ALQQEFDVEAIEITDDTIINQEFIHITYTIGFGEVPERTLSFINKNKNKIRGVAVSGNKVWGDNYGLAGDKLSAKFHTPLLLKFELSGTKQDLQKIIQEVQ-LIDKHNTKLDQAQ 1RLJ Chain:A ((15-135)) --FDSKTGNVQRFVNKTGFQQIRKVDEMDHVD-----TPFVLVTYTTNFGQVPASTQSFLEKYAHLLLGVAASGNKVWGDNFAKSADTISRQYQVPILHKFELSGTSKDVELFTQEVERVVTKSSAKM----

General information: TITO was launched using: RESULT: Template: 1RLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 542 37625 69.42 318.85 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 69.42 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1RLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RLJ-query.scw PDB file : Tito_Scwrl_1RLJ.pdb: