Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKTMKDIQAEVDRYIGQFKEGYFSPLAMMARLTEELGELAREVNHRYGEKPKKATEDDK---SMEEEIGDVLFVLVCLANSLDISLEEAHDRVMHKFNTRDKDRWTRKEEGK 2A3Q Chain:A ((48-124)) ---------------------EQFHQPRNLLLALVGEVGELAELFQWKSDTEPGPQAWPPKERAALQEELSDVLIYLVALAARCHVDLPQA---VISKMDT-------------

General information: TITO was launched using: RESULT: Template: 2A3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 149 361 2.42 4.88 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 2.42 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_2A3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A3Q-query.scw PDB file : Tito_Scwrl_2A3Q.pdb: