Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTIELNKRQEHILQIVKENGPITGEHIAEKLNLTRATLRPDLAILTMSGFLEARPRVGYFYTGKTGTQLLADKLKKLQVKDFQSIPVVIHENVSVYDAICTMFLEDVGTLFVVDRDAVLVGVLSRKDLLRASIGQ-QELTSVPVHIIMTR-MPNITVCRREDY-VMDIAKHLIEKQIDALPVIKDTDKGFEVIGRVTKTNMTKILVSLSENEIL 2QH1 Chain:A ((20-154)) -----------------------------------------------------------HMFM----------RVEKIMNSNFKTV----NWNTTVFDAVKIMNENHLYGLVVKDDNGNDVGLLSERSIIKRFIPRNKKPDEVPIRLVMRKPIPKV----KSDYDVKDVAAYLSENGLERCAVVDDPGR---VVGIVTLTDLSRYLSRASITDIL

General information: TITO was launched using: RESULT: Template: 2QH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -22346 -37.00 -169.29 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -37.00 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2QH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QH1-query.scw PDB file : Tito_Scwrl_2QH1.pdb: