Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVENPMVINNWHDKLTETDVQIDFYGDEVTPVDDYVIDGGEIILR-ENLERYLREQLGFEFKNAQ 2J28 Chain:V ((35-80)) --EAPLAIELDHDKVMNMQAKAEFYSEVLT----IVVDGKEIKVKAQDVQRH-------------

General information: TITO was launched using: RESULT: Template: 2J28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain V - contact count / total energy / energy per contact / energy per residue : 75 -10141 -135.21 -225.34 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain V : 0.73 3D Compatibility (PKB) : -135.21 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_2J28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J28-query.scw PDB file : Tito_Scwrl_2J28.pdb: