Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSDQKFIILDKSHEVGESWK-DRYDSLVLFTSRMYSSLPGMHL----EGEKHGFPSKNEIVAYLKKYVKKFEIP--IQLRTEVISVLKIK--NYFLI-KTNREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQYKN-SKQLAYGNVLVVGGGNSGAQIAVELSKE-RVTYLACSNKLVYFPLMIGKR--SIF-------------WWFD--------------------KL------G------VL----------------HASH---------TSIVGKFIQ------KKGDPVFGHELKHAIKQKEIILKK----RVIAAKQNEIIFKDSSTLEVNNIIWATGFRNPLCWINIKGVLDQEGRIIHHR----------GVS-PVEGLYFI-GLPWQHKRGSALLQGVGNDAEYIVKQMNGE 4AP3 Chain:A ((21-472)) SYDVVVVGAGIAGLYAIHRFRSQGLTVRAFEAASGVGGVWYWNRYPGARCDVESIDYSYSFSPELEQEWNWSEKYATQPEILAYLEHVADRFDLRRDIRFDTRVTSAVLDEEGLRWTVRTDRGDEVSARFLVVAAGPLSNANTPAFDGLDRFTGDIVHTARWPHDGVDFTGKRVGVIGTGSSGIQSIPIIAEQAEQLFVFQRSANYSIPAG----DDATRAEQKANYAERRRLSRESGGGSPHRPHPKSALEVSEEERRAVYEERWKLGGVLFSKAFPDQLTDPAANDTARAFWEEKIRAVVDDPAVAELLTPKDHAIGAKRIVLDSGYYETYNRDNVELVDLRSTPIVGMDETGIVT-TGAHYDLDMIVLATGFDAMTGSLDKLEIVGRGGRTLKETWAAGPRTYLGLGIDGFPNFFNLTGPGSPSVL-ANMVLHSELHVDWVADAIA--

General information: TITO was launched using: RESULT: Template: 4AP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1566 -26405 -16.86 -78.35 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -16.86 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_4AP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AP3-query.scw PDB file : Tito_Scwrl_4AP3.pdb: