Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKKQQYVTIKGTKNGLTLHLDDACSFDELLDGLQNMLSIEQYTDGK-GQKISVHVKLGNRFLYKEQEEQLTELIASKKDLFVHSIDSEVITKKE--AQQIREEAEIISVSKIVRSGQVLQVKGDLLLIGDVNPGGTVRAGGNIFVLGSLKGIAHAGFNGNNQAVIAASEMLPTQLRINHVLNRSPDHIQKGNEMECAYLDTDGNMVIERLQHLAHLRPDLTRLEGGM 1HF2 Chain:A ((1-206)) -------MVDFKMTKEGLVLLIKDYQNLEEVLNAISARITQMG-GFFAKGD--RISLMIENHNKHSQDIPRIVSHLRNLG-LEVSQILVG------KVQSRTTVESTGKVIKRNIRSGQTVVHSGDVIVFGNVNKGAEILAGGSVVVFGKAQGNIRAGLNEGGQAVVAALDLQTSLIQIAGFITHSKGEE---NVPSIAHVKG-NRIVIEPFDKVSF------------

General information: TITO was launched using: RESULT: Template: 1HF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1053 -2505 -2.38 -12.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -2.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_1HF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HF2-query.scw PDB file : Tito_Scwrl_1HF2.pdb: