Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPRDALISKNRV---KLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKL-ETEDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEGGCQVPIAGYSVL-NGQDEIEMTGLVASPDGKIIFKETVTG-------------NDPEEVGKRC------------------AALMADKGAKDLIDRVKRELDEDGK 3ECR Chain:A ((21-359)) -MRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAM-------------------LFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGW-HNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLE--CSVPVAVHTAMKDGQ--LYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILD-VARQ------

General information: TITO was launched using: RESULT: Template: 3ECR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1471 -8399 -5.71 -29.78 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -5.71 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_3ECR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ECR-query.scw PDB file : Tito_Scwrl_3ECR.pdb: