Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIMKEAIIATHNPGKVKEFKEILEPRGYDVKSLAEIGFTEEIEETGHTFEENAIMKAEAVAKAVNKMVIADDSGLSIDNLGGRPGVYSARYAGEQKDDQANIEKVLSELKGIEKEQRTARFRCALAVSIPGEETK-TVEGHVEGYIAEEPRGEYGFGYDPIFIVKDKDKTMAELTSDEKNKISHRADALKKLSKLLEA 3S86 Chain:A ((20-200)) --------LATTNPHKVEEIK-MIAPEWMEILPSPE---KIEVVEDGETFLENSVKKAVVYGKKLKHPVMADDSGLVIYSLGGFPGVMSARFM----EEHSYKEKMRTILKMLEGKDRRAAFVCSATFFDPVENTLISVEDRVEGRIANEIRGTGGFGYDPFFIPDGYDKTFGEI-PHLKEKISHRSKAFRKLFSVLE-

General information: TITO was launched using: RESULT: Template: 3S86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -6404 -6.91 -35.58 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -6.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_3S86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3S86-query.scw PDB file : Tito_Scwrl_3S86.pdb: