Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQDYSSMMYWAGKHLARKFPLES--------WEEFPAFFEEAG--WGTLTNVSAKKQELEFELEGPII-SNRLK-HQKEP-CFQLEAGFIAEQIQLMNDQIAESYEQVKK--RADKVVLTVKWDMKDPV 3TFG Chain:A ((50-183)) ----------------------HLVGA--ASEVLGKPAEEWWIAFGEYWVTYTSEEGYGELLASAGDSLPEFMENLDNLHARVGLSFPQLRPPAFECQHTSSKSMELHYQSTRCGLAPMVLGLLHGLGKRF-QTKVEVTQTAFRETGEDHDIFSIKYED----

General information: TITO was launched using: RESULT: Template: 3TFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 379 2815 7.43 23.65 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.43 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_3TFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TFG-query.scw PDB file : Tito_Scwrl_3TFG.pdb: