Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAERGASPKQIKPHRYYLLAVH 2R18 Chain:A ((126-136)) ----HRGPSPGQLKY---------

General information: TITO was launched using: RESULT: Template: 2R18.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 8 196 24.44 17.77 target 2D structure prediction score : 1.00 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 24.44 2D Compatibility (Sec. Struct. Predict.) : 1.00 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.873

(partial model without unconserved sides chains): PDB file : Tito_2R18.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R18-query.scw PDB file : Tito_Scwrl_2R18.pdb: