Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNDILEFNKTFTEQREYEKYQTSKFPDKKMAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVCVIGHHDCGMSKISSKSMLEKIKARGIPEERIETIKYSGVDFDQWFKSFDSVEASVKDSVDVIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGYNN 1YLK Chain:A ((35-168)) ----------------------------PSKHIAIVACMDARLD--VYRMLGIKEGEAHVIRNAGCVVTD---DVIRSLAISQRLLGTREIILLHHTDCGMLTFTDDDF-----KRAIQDE-------TGIRPTWSPESYPDAVEDVRQSLRRIEVNPFVTKHTSLRGFVFDVATGKLN--------

General information: TITO was launched using: RESULT: Template: 1YLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 592 -93115 -157.29 -694.88 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -157.29 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_1YLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YLK-query.scw PDB file : Tito_Scwrl_1YLK.pdb: