Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSENEQFSQMEAKARARMKERGVEVSDIAELVFFLQKKYHPDLTIDECTLNVNRVLAKREVQNAILTGIELDVLAEQKKLSEPLQTM--------LEIDESLYGVDEVLAFSIVNIYGSIGFTNYGYIDKEKPGILKRLNDKSTGECHTFLDDIVGAISAAASSRLAHRARHTE 3EA5 Chain:B ((540-649)) ----------------------------ASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAA-VIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVF-YAISALAASLGKGFEKYLETFSPYLLKALN----------------------------------

General information: TITO was launched using: RESULT: Template: 3EA5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 306 -34046 -111.26 -333.78 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -111.26 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_3EA5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EA5-query.scw PDB file : Tito_Scwrl_3EA5.pdb: