Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAA-DVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKI-MIPLRIGRASVYSQNGY-SYLIQAVKNEKGKAKYDGAGIIKR-G-SNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 3N54 Chain:B ((1-344)) ------------------------DIEQLSFARGLAIDETNDHQYKLTYQNLLPQS------GKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARG-NAADVFTKGNP-----------------------KIMIPLRIGRASVYSQNGYSYLIQAV-KNE--KGKAKYDGAGIIKRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTE-DQN----FSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIIN------

General information: TITO was launched using: RESULT: Template: 3N54.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1277 44927 35.18 146.82 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : 35.18 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_3N54.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N54-query.scw PDB file : Tito_Scwrl_3N54.pdb: