Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELERLATLFVTRF-GVEKIRLTGGEP-LMRKDMPELIKKLARIPGIRDI----AMTTNGSLL-PVYAKRLKEAGLKRVTISLDS-LEDERFKKINGRGV-SVSKVLEGIEAAKQAGLGVKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGEVASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELSDDELSEMIGTVWKNRIDQYSVDRTLSKASGKKKVEMSYIGG 4K38 Chain:A ((3-194)) --------------PLSLLIKPASSGCNLKCTYCFYHSL--SDNRNVKSYGIMRDEVLESMVKRVLNEANGHCSFAFQGGEPTLAGLEFFEKLMELQRKHNYKNLKIYNSLQTNGTLIDESWAKFLSENKF-LVGLSMDGPKEIHNLNRKDCCGLDTFSKVERAAELFKKYKVEFNILCVVTSNT-ARHVNKVYKYFKEKD--FKFLQFINC------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4K38.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 851 5160 6.06 28.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 6.06 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_4K38.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K38-query.scw PDB file : Tito_Scwrl_4K38.pdb: