Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLHKERRIGRLSVLLLLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI 1WPU Chain:A ((1-147)) -TLHKERRIGRLSVLLLLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVIAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI

General information: TITO was launched using: RESULT: Template: 1WPU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -99256 -142.61 -675.21 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -142.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.646

(partial model without unconserved sides chains): PDB file : Tito_1WPU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WPU-query.scw PDB file : Tito_Scwrl_1WPU.pdb: