Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTRALMEGAILISLFAIITL-LVVYVPVIGTILLFALPLPMILYTIRHGLKLGIWMGAVSLPVVFIVGS-F-NGLIVAFMSACAGIAMGHFFKRKEPGHAIISGALIYMLSIVFYFVISIQFLGINIIDEAMTQYRQSLDIVETVAKQSGNAGQFEKQLKLMEEQLGIVQYLFPTAIVMVGVIFSFLSYLIAKPLLRRFSPDIPNLKPFRELKFPQSVVVLYLIIVMLSFLPLEKGQMLYSIALNGEFILGFLIFIQGLSFIFFYCHKKQYPKAAAVIAVILGFVHPVFMAAIRILGVLDMGFHIRNKVK 4Z7F Chain:A ((27-124)) --GTKSIALMGVLIAVVVVFSRFFAY--ET-TFLKISFTFIPESLIGMIFGPFWAGIGTAVADVVGMLLFPKAGYFPGFTLNAFLAGAIYGYFYYKKE----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Z7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 337 -58249 -172.84 -647.21 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -172.84 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_4Z7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z7F-query.scw PDB file : Tito_Scwrl_4Z7F.pdb: