Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMKMTHLNMKDFNKPNEPFVVFGRMIPAFENGVWTYTEERFSKPYFKQYEDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIED------------IAVAKDYRKKGVGTALLHKAIEWAKENHFC-GLMLETQDINISACHFYAKHHFIIGAVDTMLYSNFPTANEIAIFWYYKF 5C88 Chain:A ((43-144)) --------------------------------------------------------VEHLKEYGLAFFVAIVDNSVVGYIMPRIEW-GFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNF---------------------------

General information: TITO was launched using: RESULT: Template: 5C88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 -15476 -46.47 -173.89 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -46.47 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_5C88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C88-query.scw PDB file : Tito_Scwrl_5C88.pdb: