Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNNLKQRRIILDLAVTLDGFIEGKNGEVDWCIMDPDMGFTDFLNQIDTILYGRKSFDLWGQYIPKNEDPDTEKELWKLVHSKKKYVFSRTQNEIDNQAIFINDNILEEVNKLKKNPGKDIWLYGGASLITTFINLGLVDEFRLSIHPVVLGEGKPLFIDVKQRINLKMVNTRTFSSGVVQIVYHWNG 1D1G Chain:A ((2-149)) --------KVIFVLAMDVSGKI--ASSVESWSSFEDRKNFRKITTEIGNVVMGRITFEEIGRPLP---------ERLNVVLTRR----PKTSN--NPSLVFFNGSPADVVKFLEGKGYERVAVIGGKTVFTEFLREKLVDELFVTVEPYVFGKGIPFFDEFEGYFPLKLLEMR---------------

General information: TITO was launched using: RESULT: Template: 1D1G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 630 -99542 -158.00 -672.58 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -158.00 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_1D1G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D1G-query.scw PDB file : Tito_Scwrl_1D1G.pdb: