Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTIFKQKNTHPFSNAANRLDRLPISRVHFQVLTALGIVYFFDLADLFTLSNVAPALIEHW----GIPLSTIANVTAASFLGMFLGASLGGRLSDRIGRK----KALNLFVFVFSIASLCNAAAWDIPSLMTF----RFLTGFGVAAAMVITNSYLAEFFPSSVRGKYISFC-------AMIGLIGVPITNIVSAFVIPLGS-------WGWRLVF---VWGAVGLIYFFFIHRLEESPRWHENRGEYAKADAILTRIEEQVEKEKGPLPAASQPKVSETVKQNAGYAGLLKG--------------RNLKITIVLSAVWIFETFGFYGFASWVPSLLKSNGVTMENTLWYNVLHSVG------APLGALLGSMIS---------ERFQRKWILAASAFLTAIAGLLYGMTFIPIMIIVFGFIVNITERVFTSNLYAYTSEPYPTEYRSSGSGLAYGLGRFSNIFGSLLVGFIAVQLGYISVF-LFIGGCWLACSLLLIFFGPNTNAKQI 4J05 Chain:A ((41-494)) ------------------------------------GVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKEL-ILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVT--ISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQR---------------------------------------------------------------WQEFVAYFSTWNHFRNLLGSML---GWFLVDIAFYGINLNQSVVLAQIGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTT-FIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIGTSAVLWIFFSTCILG-----------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1455 -189691 -130.37 -578.32 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -130.37 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: