Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVNTVIAFIIIFGT--LVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVGGFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDIVSAVKENPEKEMDVAVKRDN-------KTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVTGQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFIGVAFLMLLMLVVTWNDIQRLFL 4TN3 Chain:A ((27-400)) ------VDHCARHGEKLLLFCQEDSKVIC-----WLCKDSQEHRGHHTFLMEEVAQEYHVKLQTALEMLRQKQQEAEKLEAD-------IREEKASWKIQIDYDKTNVSADFEQLREILDWEESNELQNLEKEEEDILKSLTKSET-------EMVQQTQYMRELISELEHR-------------------LQMMDLLQGVDGIIKRIENMTLFRAPDLKGMLDMFRDAAAEESPVLLAMNIFEMLRIDEGLRLKIYKNTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTN--------------GVITKDEAEKLFNQDVDAAVRGILRNAKL---KPVYDSLDAVRRAALINM-VFQMGETGVAGFTNSLRMLQ--QKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTG------TWDAYKNL--

General information: TITO was launched using: RESULT: Template: 4TN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 841 -87722 -104.31 -250.63 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -104.31 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.078

(partial model without unconserved sides chains): PDB file : Tito_4TN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TN3-query.scw PDB file : Tito_Scwrl_4TN3.pdb: