Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGFVGAGSMAEAMINGILQSGITKPEHIYITNRSNDERLIELKETYSVRPCRDKNEFFTHTDIIILAFKPKDAAESIDSIRPYIKDQLVISVLAGLTIETIQHYFGRKLAVIRVMPNTSAAIRKSATGFSVSTEASKNDIIAAKALLETIGDATLVEERHLDAVTAIAGSGPAYVYRYIEAMEKAAQKVGLDKETAKALILQTMAGATDMLLQSGKQPEKLRKEITSPGGTTEAGLRALQDSRFEEAIIHCIEETAKRSAEIKEQFAGAALERHS 2AMF Chain:D ((5-256)) --KIGIIGVGKMASAIIKGLKQT-----PHELIISGSSLERSKEIAEQLALPYAMSHQDLIDQVDLVILGIKPQ-LFETV--LKPLHFKQPIISMAAGISLQRLATFVGQDLPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTALAGSSPAYIYLFIEALAKAGVKNGIPKAKALEIVTQTVLASASNLKTSSQSPHDFIDAICSPGGTTIAGLMELERLGLTATVSSAIDKTIDKA----------------

General information: TITO was launched using: RESULT: Template: 2AMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1036 -141855 -136.93 -562.92 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -136.93 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2AMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AMF-query.scw PDB file : Tito_Scwrl_2AMF.pdb: