Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINSFENYPMSWKPSIDKAEKPIYKALAGQLEQDILNGVLLPGTKLPPQRELADYLDLNVSTISKAFKVCELKGLLSATVGSGTFVSYDALSNAYLLEDTKPTHLIEMGAILPDHASYEP--LLYQLKNMVHEEDYEKWFSYGRAGESLWQKDAAVKLIQR-GGFETSVDHILFANGGQNAIAATLASLCKPGDRIGVDHHTYPGLKTVASLLSVQIVPIKSENDEMSPESLEYACKN-----ENIKGLYLIPDYHNPTTSFMSVENRRMVADIVKKYNLFVIEDASYHLLNKNP--LPALASFA-PQQVIHIASLSKSLAPGLRLAYVAVPRQYKEPISKALYNLNITVSPLLAELTARTIVSNQFE-VLIESHREQTIRRNQLVNRYVAGY--T--CLGVETGIFRWLLLPGKMSGAEFEELA-ARLGVQVYAAERFVVGNSCPERAVRVSVCAPKTLEELEQGLMILRRLLDDLT 2ZC0 Chain:A ((33-404)) ------------------------------------------------------------------------------------------------------GVKLISLAAGDPDPELIPRAVLGEIAKEVLEKE--PKSVMYTPANGIPELREELAAFLKKYDHLEVSPENIVITIGGTGALDLLGRVLIDPGDVVITENPSYINTLLAFEQLGAKIEGVPVDNDGMRVDLLEEKIKELKAKGQKVKLIYTIPTGQNPMGVTMSMERRKALLEIASKYDLLIIEDTAYNFMRYEGGDIVPLKALDNEGRVIVAGTLSKVLGTGFRIGWIIAEGEILKKVLMQKQPIDFCAPAISQYIALEYLKRGYFEKYHLEGALLGYKEKRDIMLKALENHLPNAEFTKPIAGMFVMFFLPEGADGISFANELMEREGVVVVPGKPFYTD-ESGKNAIRLNFSR-PSKEEIPIGIKKLAKLYKEK-

General information: TITO was launched using: RESULT: Template: 2ZC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2072 -219231 -105.81 -617.55 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -105.81 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_2ZC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZC0-query.scw PDB file : Tito_Scwrl_2ZC0.pdb: