Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKGKTIYVRPLEVTDAEENLGLQ---SENRDFFEQFSMIRADDYYTVEGQRKRITEYQERLEKDEEYHFGIFTASDDRLIGTVSLFQIIRGALQTAFIGYFLDKAHNGKGIMTEAVRLVVDYAFHELKLHRIEAGVMPRNLGSMRVLEKAGFHKEGIARKNVKINGVWEDHQVLAILNPDDEQ--- 4YGO Chain:D ((2-173)) ---NSQLTLRALERGDLRFIHNLNNNRNIMSYWFEEPY-----------ESFDELEELYNKHIHDNAERRFVVEDAQKNLIGLVELIE-INYIHRSAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEGHLVEEFFINGRYQDVKRMYILQSKYLNRSE

General information: TITO was launched using: RESULT: Template: 4YGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 692 -82387 -119.06 -496.31 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.70 3D Compatibility (PKB) : -119.06 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_4YGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YGO-query.scw PDB file : Tito_Scwrl_4YGO.pdb: