Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLCTHSLPKEKMPYLLRSGEGERYLFGRQVATVMANGRSTGDLFEIVLLSGGKGDAFPLHVHKDTHEGILVLDGKLELTLDG----ERYLLISGDYANIPAGTPHSYRMQS-H-RTRLVSYTMKGN----VAHLYSVIGNP---YDHAEHPPYASEE-V--SNERFAEAA-A--VADIVFLD---EAKPA--CSAK--L-AELTELPDGAVPYVLESGEGDRLL-TGDQLHRIVA--AQKNTDGQFIVVSSEGPKGDRIVDHYHEYHTETFYCLEGQMTMWTDG------------------QEIQLNPGDFLHVPANTVHSYRLDSHYTKMVGVLVPGLFEPFFRTLGDPYEGHIFPCEPQALRFDRILQNIEALDLKVMKP 2EA7 Chain:A ((59-357)) -------------------------------------------ENYRVVEFKSKPNTLLLPHHAD-ADFLLVVLNGTAVLTLVNPDSRDSYILEQGHAQKIPAGTTFFLVNPDDNENLRIIKLAIPVNNPHRFQDFFLSSTEAQQSYLRG-FSKNILEASFDSDFKEINRVLFGESREEGVIVELKREQIQELMKHAKSSSRKELSSQ---DEPFNLRNS-KPIYSNKFGRWYEMTPEKNPQLKDLDVFISSVDMKEGALLLPHYSSKAIVIMVINEGEAKIELVGLSDQQQQKQQEESLEVQRYRAELSEDDVFVIPAAYPVAINA-TSNLNFFAFGIN---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1067 -11778 -11.04 -48.67 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -11.04 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_2EA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EA7-query.scw PDB file : Tito_Scwrl_2EA7.pdb: