Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDYEREEEHTTPEQPKRSKKGYFLSSLIGVIVGAVLMAFIMPYLSNEGLDTGALDQQQNNNGRESIRTVNVSVNNAVTKIVSNMSPAVVGVVNIQKSDIWGESGEAGSGSGVIYKKNDHSAYVVTNHHVIEGAS-QIEISLKDGSRVSADLVGSDQLMDLAVLRVKSD-KIKAVADFGNSDKVKSGEPVIAIGNPLGLEFAGSVTQGVISGTERAIPVDSNGDGQPDWNAEVLQTDAAINPGNSGGALLNMDGKVIGINSMKIAE-SAVEGIGLSIPSKLVIPVIEDLERYGKVKRPFLGIEMKSLSDIASYHWDETLKLPKNVTNGAVVMGVDAFSPAGKAGLKELDVITEFDGYKVNDIVDLRKRLYQKKVGDRVKVKFYR-GGKEKSVDIKLSSADQLGS 1Y8T Chain:A ((6-314)) ---------------------------------------------------------------------------GSVEQVAAKVVPSVVMLETDL-----------EEGSGIILSA---EGLILTNNHVIAAAAPKTTVTFSDGRTAPFTVVGADPTSDIAVVRVQGVSGL-TPISLGSSSDLRVGQPVLAIGSPLGL--EGTVTTGIVSALNRPVST--------NTVLDAIQTDAAINPGNSGGALVNMNAQLVGVNSAIATLQSGSIGLGFAIPVDQAKRIADELISTGKASHASLGVQVTNDK----------------DTLGAKIVEVVAGGAAANAGVPKGVVVTKVDDRPINSADALVAAVRSKAPGATVALTFQDPSGGSRTVQVTLGKA-----

General information: TITO was launched using: RESULT: Template: 1Y8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1766 -9106 -5.16 -32.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -5.16 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_1Y8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y8T-query.scw PDB file : Tito_Scwrl_1Y8T.pdb: